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Image Search Results
Journal: JBMR Plus
Article Title: 3‐(3‐Hydroxyphenyl)‐Propionic Acid (PPA) Suppresses Osteoblastic Cell Senescence to Promote Bone Accretion in Mice
doi: 10.1002/jbm4.10201
Figure Lengend Snippet: HA and PPA stimulate 35 S‐GTPγS binding to membranes from mouse osteoblastic calvarial cells transfected with GPR109A but not GPR109B. Ligand‐induced 35 S‐GTPγS binding was studied using membranes prepared from mouse fetal calvarial cells expressing mouse GPR109A ( A ) or GPR109B ( B ). Binding of 35 S‐GTPγS was determined in the presence of increased concentration of nicotinic acid, acifran, HA, or PPA. HA = hippuric acid; PPA = 3‐(3‐hydroxyphenyl)‐propionic acid.
Article Snippet: Ligand‐induced 35 S‐GTPγS binding was studied using membrane fractions prepared from mouse fetal calvarial cells stably transfected with mouse GPR109A overexpression plasmid (
Techniques: Binding Assay, Transfection, Expressing, Concentration Assay
Journal: Cell Reports Medicine
Article Title: Dietary vitamin B3 supplementation induces the antitumor immunity against liver cancer via biased GPR109A signaling in myeloid cell
doi: 10.1016/j.xcrm.2024.101718
Figure Lengend Snippet:
Article Snippet:
Techniques: Control, Staining, Recombinant, Liposomes, Cell Isolation, CCK-8 Assay, TUNEL Assay, Apoptosis Assay, Activation Assay, Software
Journal: Scientific Reports
Article Title: The effect of short-chain fatty acids on human monocyte-derived dendritic cells
doi: 10.1038/srep16148
Figure Lengend Snippet: ( a ) qPCR for SCFAs receptors on monocyte-derived DC compared to MCF-7 cell line as internal control. Shown are the averages ± standard deviations (SD) (n = 3). ( b ) Flow cytometry analysis of GPR41 and GPR109A surface expression on monocyte-derived DC and ( c ) primary CD141 + and CD1c + DC. Mean fluorescence intensity (MFI) averages ± standard deviations (SD) (n = 3) are reported below each histogram. Gates for GPR41- or GPR190A-positive cells in both figures ( b,c ) has been placed according to their respective isotype control. The different shades of grey represent cells from three different donors used for the experiments.
Article Snippet: Anti-human HLA-DR (PE, BD, clone L243), anti-human CD83 (BV421, Biolegend, clone HB15e) and anti-human CD86 (APC, Biolegend, clone IT2.2), anti-human CD141 (BV421, BD, clone 1A4), anti-human CD1c (BB515, BD, clone F10/21A3), anti-human GPR41 (APC, LSBio),
Techniques: Derivative Assay, Flow Cytometry, Expressing, Fluorescence